New Step by Step Map For thr77 login
New Step by Step Map For thr77 login
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important position in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two added splice variants ended up described but specific nucleotide sequence facts will not be yet out there.
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Blocking peptides are peptides that bind specially for the target antibody and block antibody binding. These peptide commonly consists of the epitope acknowledged via the antibody.
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This guide outlines more achievable optimization of cellular and immunoassay parameters to make sure the best possible benefits are...
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This information outlines more achievable optimization of cellular and immunoassay parameters to make sure the best possible results are...
Antibodies bound to the blocking peptide now not bind to the epitope around the concentrate on protein. By comparing the staining through the blocked antibody as opposed to the antibody by itself, you can see which staining is distinct.
Target Information and facts Cyclin E belongs to your very conserved cyclin family members, whose users are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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